Amelioration of 1, 2 Dimethylhydrazine Induced Tumor Promotion Response by Novel Benzimidazole Derivative Nanoparticle in Wistar Rats

Abstract

Background: Colon cancer is one of the most common malignancies in many regions of the world and is thought to arise from the accumulation of mutations in a single epithelial cell of the colon and rectum. The benzimidazole comprises a important pharmacophore and privileged structure in modern drug discovery. Various substituted benzimidazole derivatives have been found to possess potential anticancer properties.Objective: The study aimed to prove the anti-colon cancer activity of novel benzimidazole derivative 4-(1H-benzo[d]imidazol-2-yl)-6-phenylpyrimidin-2-amine loaded chitosan nanoparticle (BZI 3 nano) by an 1, 2 Dimethylhydrazine (DMH) Induced rat modelin-vivostudy and identify the targeting efficiency of BZI 3 nano to treat colorectal cancer.Method: The effect of novel benzimidazole derivative 4-(1H-benzo[d]imidazol-2-yl)-6-phenylpyrimidin-2-amine loaded chitosan nanoparticle (BZI 3 nano) on the formation of aberrant crypt foci (ACF), apoptosis, histopathology, body weight, organs weight and heamotological parameters were studied in 1,2-dimethylhydrazine (DMH)-induced colon cancer in rats.Results: BZI 3 nano (5 mg/kg, p.o) administration significantly reduced ACF number and increased the weight gain and apoptotic index compared to DMH treated group. The histological alterations induced by DMH were also significantly improved.Conclusion: In-vivo anticancer activities results revealed that the presence substituted benzimidazole derivative nanoparticle (BZI 3 nano) could have the anticancer potential of the scaffold and selective, good target for drug discovery, which can be regarded as promising anticancer potential.

Keywords: Benzimidazole derivative; Nanoparticles; DMH; ACF