Article · Volume 11, Issue 1 · pp. 51–55
Anti-cancer Activity and Gene Expression Responses to Methanol Extract of Gladiolus segetum in THP-1 Human Monocytic Leukemia Cells
Marref Salah Eddine1, Benkiki Naima1, Melakhessou Mohamed Akram1, Olivier Joubert2, Zahra Manel Doumandji2, Ramia Safar2
- 1Laboratoire de Biotechnologie des Molécules Bioactives et de la Physiopathologie Cellulaire. Université de Batna-2, 05000, ALGERIE.
- 2Faculté de Pharmacie, EA 3452 CITHÉFOR, Université de Lorraine, 5 rue Albert Lebrun, 54000 Nancy, FRANCE.
Abstract
Objective: To Evaluate the viability of cells against exposure to the Methanol Extract of Gladiolus segetum (MEGS) by various colorimetric detection tests (Alamar Blue, WST-1) and the trypan blue exclusion test in Human Leukemia Monocytes THP-1 and Using reverse transcription-polymerase chain reaction assays.Methods: Human THP-1 monocytic cell line was exposed to 25 to 800 μg/mL concentrations of MEGS for 24 h and cellular viability was estimated using Alamar Blue, WST-1 and trypan blue tests. Gene expressions were performed using reverse transcription-polymerase chain reaction assays and the measurement of Caspase-3 enzyme activity.Results:A decrease of viability was observed with a dose-dependent effect of MEGS on THP-1. In addition, a differential expression involved in the different genes tested. Moreover, it has been shown overexpression of casp3 for an exposure to 25 μg/ml and 100 μg/ml of MEGS.Conclusion: Our results show that the MEGS present a toxicity to THP-1 cells, especially with regard to apoptosis processes.
Keywords: Gene expressions; Viability; Gladiolus segetum; THP-1; Toxicity
The full text of this article is available as a PDF. Open the PDF.