Article · Volume 11, Issue 4 · pp. 395–398
Influencing Effect of Heat Therapy on Osteoblasts Growth and Differentiation Following Treatment with Bone Antiresorptive Drugs- An in vitro Study
Alqhtani NR1, Adel Alenazi1, Fazil Arshad Nasyam1, Sajeda Mehaji2
- 1Department of Oral and Maxillofacial Surgery and Diagnostics Sciences, College of Dentistry, Prince Sattam Bin Abdul Aziz University, Alkarj, SAUDI ARABIA.
- 2Department of Emeritus Reader in Oral Biology, Eastman Dental Institute University College-London, London, UNITED KINGDOM.
Abstract
Objectives: To find out the effect of mild heat shock on osteoblast proliferation and mineralisation following treatment with bisphosphonates.Materials and Methods: Cell culture of MC3T3 cells was performed in alpha minimum essential medium supplemented with 10% Foetal Calf Serum (FCS), 2 mmol L-glutamine and 100U/ml each of penicillin/streptomycin and were incubated at 37°C in humidified 5% CO2 . The selected bisphosphonate drugs were administered, following which heat therapy was given by placing the flasks in a temperature-regulated water bath. The proliferation of osteoblast progenitors was measured using the methylene blue staining technique.Results:The results showed that mild heat-shock for 2 min at 42°C can stimulate proliferation of osteoblast progenitors, as well as bone nodule formation. In addition, mild heat-shock can reverse the action of bisphosphonate by stimulation of osteoblast proliferation and mineralisation.Conclusion: Mild heat-shock enhances cell differentiation and osteoblasts mineralisation following bisphosphonate treatment.
Keywords: Bisphosphonates; Heat-shock; Osteoblast; Osteoclast
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