Assessment of Anthelmintic Activity and in silico Study of Phytoconstituents in Decaschistia crotonifolia Wight & Arn. Root Extract

Abstract

Background: Worm infections in developing countries were reported high. Phytoconstituents have been a vital role for the treatment of many ailments. The current study was aimed assess for anthelmintic activity of different root extracts of Decaschistia crotonifolia belongs to the family Ebanaceae against Pheretima posthuma. Further Insilico study was carried out for phytocompounds present in Dechaschistia.Methods: The chloroform, ethylacetate and ethanol extract of Decaschistia crotonifolia were considered for the study of anthelmintic property on earthworms at concentrations 20 mg/ml, 40 mg/ml and 60 mg/ml. During this study, the parameters paralysis time and Death Time of adult Indian earthworms was observed. As a standard and control Albendazole 10 mg/ml and 2% Tween 80 in distilled water were taken respectively.Results: The study resulted that ethanolic extract was significant when compared with the Albendazole 10 mg/ml. Docking studies revealed all phytocompounds in Dechaschistia shown binding affinity, however comparatively scopoletin and stigmasterol had shown a good binding affinitiy about -7.7 Kcal/mol and -7.6 Kcal/mol compared to standard drug Albendazole which was shown about -8.7 Kcal/mol.Conclusion: The study revealed that the ethanol extract of Decaschistia crotonifolia at a concentration of 60mg/ml exhibited a stronger anthelmintic property compared to Albendazole 10mg/ml. A dose dependent anthelmintic activity is exerted by all the extracts in an ascending manner Chloroform

Keywords: Decaschistia crotonifolia Wight and Arn; Ebanaceae; Pheretima and Anthelmintic; Docking; Lipinski rule