Neuroprotective Effect of Diplocyclos palmatus on Aβ (25-35) Induced Alzheimer’s Disease in Mice

Abstract

Objectives: Alzheimer's disease is primarily caused by neurotoxic effects of amyloid beta Aβ(25-35) peptide accumulation and increased levels of Acetylcholinesterase Enzyme (AChE). Acetylcholinesterase inhibitors report for the effective management of cognitive and motor disorders. In the current study the impact of Diplocyclos palmatusMethanolic (DPM) seed extract and its Chloroform (DPC) fractions were investigated in mice with Amyloid beta (Aβ)-induced experimental Alzheimer's disease.Materials and Methods: Acute toxicity study was performed based on the guidelines of OECD 423 and doses were selected. Mice were administered with standard Donepezil (5 mg/kg/oral) and two doses each of DPM and DPC daily for 21 days (200 mg/kg and 400 mg/kg/oral). Aβ was given by Intra Cerebro Ventricular (ICV) injection in a single dose 3 mg/kg. Cognitive abilities were assessed using the conditioned avoidance test, the rectangular maze and Y-maze. On 22nd day mice were sacrificed, then isolated brain homogenate used for estimation of biochemical parameters such as reduced Glutathione peroxidase (reduced GSH), Malondialdehyde (MDA), nitrite level and AChE levels.Results: Administration of DPM and DPC extracts effectively reduces behavioral and biochemical abnormalities in dose dependent way.Conclusion: Diplocyclos palmatusseeds showed neuroprotective effect on Aβ-induced AD in mice due to their antioxidant and AChE activity.

Keywords: Alzheimer’s disease; Diplocyclos palmatus; Acetylcholinesterase