Evaluation of Topical Anti-Inflammatory Potential of Mentha piperita L. Extract by Formulation of Microemulgel

Abstract

Background: The present study is designed to develop a novel dosage form i.e. microemulgel which will enhance the rate of absorption in the systemic circulation and ultimately enhance the pharmacological effect of the Mentha piperitaL. extract as anti-inflammatory agent. Its primary components include with constituents including menthol (46.32%), menthofuran (13.18%), menthyl acetate (12.10%), menthone (7.42%) and 1,8-cineole (6.06%).Materials and Methods: The research aimed to formulate and assess herbal microemulgel containing M. piperitaextract, focusing on its in vitroanti-inflammatory properties. M. piperitaherb extraction was carried out using a hydro-alcoholic solvent, followed by phytochemical analysis. Four separate sets of herbal microemulgel were crafted and underwent a series of assessments, encompassing pH levels, spreadability, viscosity, consistency, appearance, color and ease of washing. Additionally, the in vitroanti-inflammatory potential of both the extract and the microemulgel formulation was assessed using the HRBC membrane stabilization assay and the protein denaturation assay.Results: The findings of this study suggest that the newly developed herbal microemulgel, enriched with M. Piperitaextract, exhibits promising anti-inflammatory effects.Conclusion: The M. piperitamicroemulgel exhibited a remarkable 94.35% drug content with high solubility and compatibility of the drug with the excipients. Permeability studies revealed that the M. piperitamicroemulgel achieved 94% permeability within 48 hr, showcasing enhanced drug permeability facilitated by the microemulsion-based gel system. Moreover, the formulated microemulgel demonstrated significant anti-inflammatory activity. It can be concluded that topical herbal M. piperitamicroemulgel has potential for future applications in this regard.

Keywords: M. piperita; Menthol; Herbal microemulgel; Anti-inflammatory activity; HRBC membrane; Deodarin