Biological Profiling and Phytochemical Analysis of Peristrophe bicalyculata: Evaluating its Therapeutic Potential and Active Compounds

Abstract

Background: The present study deals the biological property of a medicinal plant, Peristrophe bicalyculata. This is a common herb used for a number of ailments ethno-pharmacologically. In the present study, the antioxidant, anti-inflammatory and anti-cancer activity of the ethyl acetate extract of P. bicalyculatawas reported.Materials and Methods: After collecting the plants from nearby hills of Chengalpattu, Tamil Nadu, the aerial part was washed and dried. The ethyl acetate extract of the plant was prepared using maceration method studied for its anti-oxidant, anti-inflammatory and cytotoxic activities.Results: Results revealed that the extract can able to scavenge the radicles at the IC50 value of 33.96 μg/mL. Similarly, the anti-inflammatory activity also revealed that IC50 value of protein denaturation assay and HRBC membrane stabilization assay was 125 μg/mL and 41.5 μg/mL respectively. In lung cancer cell line A549, 62.57% of the cell death was observed at 1000 μg/mL. Ao/EtBr staining reveals cell death is mainly due to apoptosis and necrosis was less observed.Conclusion: The study concludes that the plant has potential anticancer activity. Further studies on the bioactive compound behind the activity and their mechanism will provide a clear insight.

Keywords: Peristrophe bicalyculata; Antioxidant; Anti-Inflammatory; Anticancer; Molecular Docking