Article · Volume 3, Issue 1 · pp. 55–59
A Validated Densitometric Method for Analysis of Atorvastatin Calcium and Metoprolol Tartarate as Bulk Drugs and In Combined Capsule Dosage Forms
Patole SM1, Khodke AS1, Potale LV1, Damle MC2
- 1Department of Quality Assurance, A.I.S.S.M.S. College of Pharmacy, Kennedy Road, Near R.T.O., Pune, INDIA.
- 2Assistant Professor, Department of Pharmaceutical Chemistry, A.I.S.S.M.S. College of Pharmacy, Kennedy Road, Near R.T.O., Pune, INDIA.
Abstract
A simple, accurate and precise high-performance thin-layer chromatographic method has been developed for the estimation of Atorvastatin Calcium and Metoprolol Tartarate simultaneously from a capsule dosage form. The method employed Silica gel 60F254s precoated plates as stationary phase and a mixture of Chloroform: Methanol: Glacial acetic acid (dil.) :: (9:1.5:0.2 ml %v/v) as mobile phase. Densitometric scanning was performed at 220 nm using Camag TLC scanner 3. The method was linear in the drug concentrations’ range of 500 to 2500 ng/spot for Atorvastatin Calcium, also for Metoprolol Tartarate with correlation coefficient of 0.984 for Atorvastatin Calcium and 0.995 for Metoprolol Tartarate respectively. The retention factor for Atorvastatin Calcium was 0.45 ± 0.04 and for Metoprolol Tartarate was 0.25 ± 0.02. The method was validated as per ICH (International Conference on Harmonisation) Guidelines, proving its utility in estimation of Atorvastatin Calcium and Metoprolol Tartarate in combined dosage form.
Keywords: Atorvastatin calcium; high performance thin layer chromatography; metoprolol tartarate; validation
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