Communication · Volume 5, Issue 3 · pp. 98–101

Simple and sensitive method for the analysis of ketorolac in human plasma using high-performance liquid chromatography

Selvadurai Muralidharan¹, Kalaimani Jayaraja kumar², Subramani Parasuraman³

¹Unit of Pharmaceutical Chemistry, Faculty of Pharmacy, Asian Institute of Medicine, Science and Technology (AIMST) University, Bedong 08100, Kedah, MALAYSIA.
²Unit of Pharmaceutical Technology, Faculty of Pharmacy, Asian Institute of Medicine, Science and Technology (AIMST) University, Bedong 08100, Kedah, MALAYSIA.
³Unit of Pharmacology, Faculty of Pharmacy, Asian Institute of Medicine, Science and Technology (AIMST) University, Bedong 08100, Kedah, MALAYSIA.

Abstract

Objective: To develop a simple and sensitive method of ketorolac in drug free human plasma using high-performance liquid chromatographic (HPLC).Method: Ketorolac from blank plasma was extracted using protein precipitation method. Trichloro acetic acid (10%) was used as a protein precipitating agent and the percentage of recovery was calculated by adding known volume of dexibuprofen as internal standard. The HPLC separation was achieved on reversed-phase C18 column and the separations were detection by Photodiode Array (PDA) detector at 306 nm. Acetonitrile and 5 mM ammonium acetate (pH 3.5), in the ratio of 60:40% v/v was used as mobile phase at the isocratic flow rate of 1.0 ml/min. The method was validated according to FDA Guidance for Industry.Result: The percentage of recovery of ketorolac was 93.7(+-)0.12 (mean(+-)SD; n ¼ 5). The linearity was achieved in this method range of 10.0e125.0 ng/ml with regression coefficient range of 0.999.Conclusion: Ketorolac bioanalytical method was developed and validated according to FDA Guidance for Industry.

Keywords: Dexibuprofen; Ketorolac; Pharmacokinetics

The full text of this article is available as a PDF. Open the PDF.