Nephroprotective Effect of Pterocarpus indicus Willd. Leaves: Observation From Plasma Urea and Creatinine Levels Against Gentamicin-Induced Nephrotoxicity in Sprague-Dawley Rats

Abstract

Introduction: Pterocarpus indicus Willd. has been widely used empirically for treating canker sore, antibacterial and kidney disease.Objective: This study aimed to evaluate nephroprotective effect water-boiled P. indicus leaves against gentamicin-induced nephrotoxicity in rats observed from plasma urea and creatinine levels.Materials and Methods: In this study, thirty (30) male rats divided into five treatment groups. The treat ment groups consisted of the normal group, induction group received gen tamicin (80 mg/kg body weight per day i.p.) and a group which received water-boiled P. indicus leaves with a dose of 28.8 mg/kg, 57.6 mg/kg, and 115.2 mg/kg body weight per day p.o. All groups received treatment for 21 days. At the 15th day, the animals received gentamicin i.p. for 7 days. At the 22nd day, the blood was collected from orbital sinus. The plasma urea and creatinine levels were measured by Berthelot method for plasma urea and colorimetric method for plasma creatinine.Results: The highest dose of 115.2 mg/kg water-boiled P. indicus was decreased plasma urea and creatinine levels also significantly different (p<0,05) compared induc tion group.Conclusions: Water-boiled P. indicus leaves have a potential to prevent gentamicin-induced nephrotoxicity might be due to its antioxidant and free radical scavenging activity.

Keywords: Gentamicin; Nephroprotective; Plasma creatinine; Plasma urea; Pterocarpus indicus Willd