Article · Volume 12, Issue 2 · pp. 40–43
Phytochemical Screening and Haem Polymerization Inhibitory Activity of Root Extract and Fractions from Strychnos lucida R. Br.
Uswatun Khasanah1, Novia Ariani2, Yumna Nadya Aprilia2, Sri Winarsih1
- 1Department of Pharmacy, Faculty of Medicine, Brawijaya University, Malang, East Java, INDONESIA.
- 2Undergraduate Student of Pharmacy Program Study, Faculty of Medicine, Brawijaya University, Malang, INDONESIA.
Abstract
Background: Strychnos lucida R. Br. is used empirically by people in Eastern Indonesia to treat malaria symptoms. The Indonesian government has set a decrease in malaria morbidity as a long-term target. This study aimed to determine haem polymerization inhibitory activity and screen secondary metabolites of root extract and fractions from S. lucida.Materials and Methods: Screening of secondary metabolites of root extract and fractions were performed using TLC. Crude extract was fractionated using dichloromethane (DCM), ethyl acetate (EA), and N-butanol as solvents. Measurement of haem polymerization inhibition was carried out in vitro using β-haematin.Results: The DCM fraction showed the most effective haem polymerization inhibitory activity (IC50 of 8.96 mg/ml), followed by the n-butanol, aquadest, ethyl acetate, and crude extract. The IC50 values for the DCM, n-butanol, aquadest, and ethyl acetate fractions were smaller than the chloroquine diphosphate. Based on the results of secondary metabolite screening, the DCM fraction of S. lucida root contains terpenoids, alkaloids, and tannins.Conclusion: The content of secondary metabolites influenced the inhibitory activity of haem polymerization in each extract and fraction.
Keywords: Strychnos lucida R. Br; β-haematin; Phytochemical screening; Hemozoin; Fraction; Antimalarial
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