Article · Volume 4, Issue 2 · pp. 29–33
Cold Extraction of Carissa congesta Wight monitored by a comparative revision of HPLC and HPTLC
Gaurav M. Doshi1,2, Pratip K. Chaskar1, Sandeep Zine1, Hemant D. Une3
- 1Assistant Professors, Department of Pharmacology and Pharmaceutical Chemistry, Vivekanand Education Society’s College of Pharmacy, Mumbai,, INDIA.
- 2Pacific Academy of Higher Education and Research University, Udaipur, Rajasthan, INDIA.
- 3Associate Professor, Vice Principal, Department of Pharmacology, Y. B. Chavan College of Pharmacy, Rouzabagh, Aurangabad, Maharashtra, INDIA.
Abstract
Background: Carissa congesta is an important medicinal plant in India. Lupeol, an active constituent of this species, has been reported to possess good amount of pharmacological as well as therapeutic potential.Objective: In the current studies enlighten we focussed on determining the percentage of the lupeol present in the plant root extract by sophisticated analytical techniques.Materials and Methods: Shade-dried roots of Carissa congesta were subjected to a cold petroleum ether extraction followed by monitoring the lupeol contents by HPTLC and HPLC methods after carrying out preliminary phytochemical screening for the active constuients.Results: The extraction yield was found to be 1.8% (w/w). Phytochemical screening of the extract showed the presence of steroids, flavonoids, saponins, glycosides, tannins, phenolic compounds, fixed oils and fats.. The amount of lupeol present was found to be 25.80 % (w/w) and 11.18 % (w/v) by HPTLC quantification and HPLC analysis respectively. From HPLC analysis, the petroleum ether extract was found to a peak which eluted coincident with lupeol, indicating that the extract contained lupeol Conclusion:The extract contains significant amount of lupeol.
Keywords: Carissa congesta; Cold Extraction; HPLC; HPTLC; lupeol
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