Protective Effect of Trichosanthes Dioica Extract Against Gentamicin Induced Nephrotoxicity in Rats

Abstract

Introduction: Nephropathy is one of the progressive complications prevailed all around the world that is observed which arises due to detrimental effects of metabolites created due to various metabolic and physiological reactions. Gentamicin is one of the drug that may cause nephrotoxicity.Objectives: Phytoremedies are being considered as to play deterrent role in the treatment of disease. T. dioica is widely distributed in Asian subcon- tinent and consumed as food. The present work is aimed to study nephro- protective activity of T. dioica.Subjects and Methods: Dried T. dioica fruits were extracted with methanol and the extract was subjected to phyto- chemical analysis. Acute toxicity was determined as per OECD guidelines. Gentamicin (80 mg/Kg) was used to induce nephrotoxicity. The extract was given orally. Kidney weight was determined and estimation of blood urea nitrogen, serum creatinine, uric acid, catalase, superoxide dismutase, glutathione peroxidase, lipid peroxidation, urinary electrolytes was performed. Results and Discussion: Decrement in serum urea and creatinine along with reduction in uric acid was observed. Similarly, restoration of antioxi- dant enzymes and normalization of urinary electrolytes and kidney weight was observed. Observations from present study divulged that T. dioica, which is a good source of antioxidant phytochemicals, is accountable for nephroprotective activity.

Keywords: T. dioica; Nephroprotective; Histopathology; Uric acid; Creatinine; Urea nitrogen