Article · Volume 1, Issue 3 · pp. 89–90

Isolation of forskolin from stem of Coleus forskohlii

A.M. Saleem^1,2, P.B. Dhasan³, M.R.M. Rafiullah⁴

¹Department of Pharmacognosy, C.L.Baid Metha College of Pharmacy, Thoraippakkam, Chennai 600096, India.
²School of Pharmacy, College of Medicine, National Taiwan University, Taipei 100, Taiwan R.O.C.
³Department of Siddha Medicine, Tamil University, Thanjavur 613500, India.
⁴Faculty of Pharmacy, Hamdard University, New Delhi 110062, India.

Abstract

Forskolin was isolated from the stem of Coleus forskohlii. Chloroform extract of the powdered stem was subjected to silica gel column chromatography to obtain crude forskolin. The crude forskolin was crystallized using ethyl acetate and n-hexane to obtain pure forskolin. The forskolin isolated was characterized by using UV, IR, ESI-Mass, 1H and 13C NMR. The yield was 0.103% w/w based on the dry weight of stem powder. The present study reconfirmed existing data on physical and spectroscopic characterization of forskolin.

Keywords: Coleus forskohlii; Stem; Forskolin; Isolation; Purification; NMR

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