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PM · Lens · Effect of different growth stages of Ziziphora clinopodioides Lam. on its chemical composition

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Article · Volume 10, Issue 37S · pp. S1–S5

Effect of different growth stages of Ziziphora clinopodioides Lam. on its chemical composition

Wenhuan Ding2, Tao Yang3, Feng Liu4, Shuge Tian2,5

  1. 1Department of Traditional Chinese Medicine, College of TCM, Xinjiang Medical University, Urumqi‑830011, China.
  2. 2Product Quality Supervision and Inspection Academy of Xinjiang, Urumqi 830004, China.
  3. 3Department of chemistry, College of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumqi‑830054, China.
  4. 4Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi‑830011, Xinjiang, China.

Abstract

Aims: The aim of this study is to monitor the changes in the chemical composition of Ziziphora clinopodioides Lam. throughout nine different growth stages.Materials and Methods: Volatile components such as essential oils were analyzed using the gas chromatography (GC) and GC‑mass spectrometry, and the contents of non‑volatile components were determined by a visible spectrophotometer.Results: Hydro‑distilled essential oil content ranged from a minimum of 1.1% (in the post‑flowering stage) to a maximum of 1.8% (in the flowering stage). The essential oils included pulegone, which was the most abundant component (77.48‑87.3%), p‑menthanone (2.79‑12.39%), trans‑isopulegone (1.04‑2.06%), d‑limonene (0.51‑3.03%) and eucarvone (1.5‑4.48%). The contents of non‑volatile components, such as that of total phenolics (TPC), total flavonoids (TFC), total triterpenoids content (TTC) and total free amino acids content (TFAAC) were measured using visible spectrophotometry. In the growing stage, TPC, TFC, TTC and TFAAC were 9.91‑12.80 mg/g, 29.84‑50.63 mg/g, 0.57‑1.41 mg/g and 13.33‑28.56 mg/g, respectively.Conclusion: These data can be used as a basis to determine the optimal harvest time of Z. clinopodioide Lam.

Keywords: Chemical composition; dynamic changes; Ziziphora clinopodioide Lam

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