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PM · Lens · Multi‑component analysis in sun‑dried and sulfur‑fumigated Angelicae Sinensis Radix by single marker quantitation and chemometric discrimination

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Article · Volume 10, Issue 37S · pp. S189–S197

Multi‑component analysis in sun‑dried and sulfur‑fumigated Angelicae Sinensis Radix by single marker quantitation and chemometric discrimination

Yajing Lou2,3, Hao Cai2,3,4, Xiao Liu2,3,4, Gang Cao3,4,6, Sicong Tu7, Songlin Li5, Xiaoqing Ma2,3, Kunming Qin2,3, Baochang Cai2,3,4,6

  1. 1Department of Chinese Materia Medica, College of Pharmacy, Nanjing University of Chinese Medicine.
  2. 2Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine.
  3. 3National First‑Class Key Discipline for Science of Chinese Materia Medica, Nanjing University of Chinese Medicine.
  4. 4Department of Pharmaceutical Analysis and Metabolomics, Jiangsu Province Academy of Chinese Medicine, Nanjing, College of Pharmacy, Nanjing University of Chinese Medicine.
  5. 5Research Center of Traditional Chinese Medicine Processing Technology, Zhejiang Chinese Medical University, Hangzhou, People’s Republic of China.
  6. 6Faculty of Medicine, University of New South Wales, Sydney, New South Wales, Australia.

Abstract

Background: A new method has been developed for the simultaneous determination of ferulic acid, senkyunolide A, and Z‑ligustilide in Angelicae Sinensis Radix before and after sulfur‑fumigation using quantitative analysis of multi‑components by a single marker (QAMS).Materials and Methods: The feasibility and accuracy of QAMS were checked by the external standard method, and various high‑performance liquid chromatographic instruments and chromatographic conditions were investigated to verify its applicability. Using ferulic acid as the internal reference substance, and the contents of senkyunolide A and Z‑ligustilide were calculated according to relative correction factors by high‑performance liquid chromatography. Meanwhile, the influence of sulfur‑fumigation on these chemical components in Angelicae Sinensis Radix were evaluated and discriminated by chromatographic fingerprint and chemometrics.Results: There was no significant difference observed between the QAMS method and the external standard method. Furthermore, sulfur‑fumigation reduced the contents of ferulic acid, senkyunolide A, and Z‑ligustilide in Angelicae Sinensis Radix by some degree, and the sun‑drying and sulfur‑fumigation processing could be easily discriminated by chromatographic fingerprint and chemometrics.Conclusion: QAMS is a convenient and accurate approach to analyzing multi‑component when reference substances are unavailable, simultaneously, chemometrics is an effective way to discriminate sun‑dried and sulfur‑fumigated Angelicae Sinensis Radix.

Keywords: Angelicae Sinensis Radix; chemometrics; quantitative analysis of multi‑components by a single marker; relative correction factor; sulfur‑fumigation

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