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PM · Lens · Qualitative and quantitative analysis of the major constituents in traditional Chinese medicine Danmu injection using LC‑ESI‑MSn and LC‑DAD

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Article · Volume 10, Issue 39 · pp. 254–264

Qualitative and quantitative analysis of the major constituents in traditional Chinese medicine Danmu injection using LC‑ESI‑MSn and LC‑DAD

Fen‑Xia Zhu1, Jing‑Jing Wang1, Xiu‑Feng Li2, E Sun1, Xiao‑Bin Jia1

  1. 1Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China.
  2. 2Engineering Centers for Li Nationality Medicine, Hainan Pharmaceutical Factory, Co., Ltd, Wuzhishan 572200, P.R, China.

Abstract

Background: Danmu injection, a traditional Chinese medicine (TCM) preparation made from Nauclea officinalis, has been commonly used for the treatment of cold, fever, swelling of throat in China. However, the chemical constituents in Danmu injection have not been clarified yet.Objective: a HPLC/DAD/ESI‑MSn method was developed for qualitative and quantitative analysis of the components in Danmu injection.Materials and Methods: The chromatographic separation was performed on a Welch Material XB‑C18 (4.6mm × 250mm, 5µm) using gradient elution with acetonitrile (A) and water containing 0.1% formic acid (B) as mobile phase at a flow rate of 1.0 ml/min.Results: Twenty‑five compounds, including phenolic acid and phenol glycoside, iridous glycoside and glycoalkaloid were identified or tentatively deduced on the base of their retention behaviors, UV absorption, MS and MSn data with those elucidated references or literature. In addition, eleven compounds were simultaneously determined by HPLC–DAD, which was validated and successfully applied for determination of major components in Danmu injection.Conclusion: The results suggested that the established qualitative and quantitative method would be a powerful and reliable analytical tool for the characterization of multi‑constituent in complex chemical system and quality control of Danmu injection.

Keywords: Danmu injection; HPLC–DAD–ESI‑MSn; major constituents; Nauclea officinalis; quality control

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