A rapid UPLC method for simultaneous determination of eleven components in ‘Ge‑Gen‑Qin‑Lian’ decoction

Abstract

Background: ‘Ge‑Gen‑Qin‑Lian’ Decoction derived from ‘Shang‑Han‑Lun’ compiled by Zhang Zhongjing. It is widely used in the treatment of acute gastroenteritis, bacillary dysentery, virus diarrhea. This paper describes a sensitive and specific assay for the determination of the 11‑marker compounds using ultra performance liquid chromatography (UPLC).Objective: To develop an UPLC method for simultaneous determination of 11 bioactive compounds in ‘Ge‑Gen‑Qin‑Lian’ preparations.Materials and Methods: The chromatography analysis was performed on an Agilent Proshell 120 EC‑C18 column (4.6 × 50 mm, 2.7 μm) at 30°C with a gradient elution of methanol, 0.5% formic acid and 0.5% ammonium acetate at a flow rate 1.0 ml/min and UV detected at 270 nm.Results: All calibration curves showed good linear regression (r ≥ 0.9993) within tested ranges. Limits of detection (LOD) and limits of quantification (LOQ) fell in the range between 0.0691-1.04 μg/ml and 0.23–3.43 μg/ml, respectively. The mean recovery of each herbal medicine ranged from 96.60 to 102.11%.Conclusion: The method was validated for repeatability, precision, stability, accuracy, and selectivity. The validated method was successfully applied to simultaneous analysis of these active components in ‘Ge‑Gen‑Qin‑Lian’ decoction.

Keywords: Determination; eleven components; Ge‑Gen‑Qin‑Lian decoction; quality evaluation; UPLC A B S T R A C T