Simultaneous determination three phytosterol compounds, campesterol, stigmasterol and daucosterol in Artemisia apiacea by high performance liquid chromatography‑diode array ultraviolet/visible detector

Abstract

Background: Artemisia apiacea is a traditional herbal medicine using treatment of eczema and jaundice in Eastern Asia, including China, Korea, and Japan.Objective: An accurate and sensitive analysis method using high performance liquid chromatography‑diode array ultraviolet/visible detector and liquid chromatography–mass spectrometry for the simultaneous determination of three phytosterol compounds, campesterol, stigmasterol and daucosterol in A. apiacea was established.Materials and Methods: The analytes were separated on a Shiseido C18 column (5 µm, 4.6 mm I.D. ×250 mm) with gradient elution of 0.1% trifluoroacetic acid and acetonitrile. The flow rate was 1 mL/min and detection wavelengths were set at 205 and 254 nm.Results: Validation of the method was performed to demonstrate its linearity, precision and accuracy. The calibration curves showed good linearity (R2 > 0.9994). The limits of detection and limits of quantification were within the ranges 0.55–7.07 µg/mL and 1.67–21.44 µg/mL, respectively. And, the relative standard deviations of intra‑ and inter‑day precision were <2.93%. The recoveries were found to be in the range of 90.03–104.91%.Conclusion: The developed method has been successfully applied to the analysis for quality control of campesterol, stigmasterol and daucosterol in A. apiacea.

Keywords: Artemisia apiacea; high performance liquid chromatography‑diode array ultraviolet/ visible detector; liquid chromatography–mass spectrometry; simultaneous determination A B S T R A C T