Article · Volume 11, Issue 42 · pp. 311–319
Effects of Pogostemon cablin Blanco extract on hypoxia induced rabbit cardiomyocyte injury
Chi‑Yeon Lim1, Bu‑Yeo Kim2, Se‑Hyun Lim3, Su‑In Cho4
- 1Department of Medicine, College of Medicine, Dongguk University, Ilsan 305‑701.
- 2Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 305‑701.
- 3Department of Nursing, School of Public Health, Far East University, Chungbuk 369‑700.
- 4Department of Korean Medicine, School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam 626‑870, Republic of Korea.
Abstract
Background: Pogostemonis Herba, the dried aerial part of Pogostemon cablin Blanco, is a well‑known materia medica in Asia that is widely used for syndrome of gastrointestinal dysfunctions.Objective: This study was undertaken to examine whether Pogostemon cablin extract (PCe) might have any beneficial effect on hypoxia induced rabbit cardiomyocyte injury.Materials and Methods: Isolated cardiomyocytes were divided into three groups and the changes of cell viability in cardiomyocytes of hypoxic and hypoxia/reoxygenation group were determined. The effect of PCe on reactive oxygen species (ROS) generation, intracellular formation of ROS was also measured by monitoring the 2’,7’‑dichlorofluorescein fluorescence.Results: PCe effectively protected the cells against both the hypoxia and reoxygenation induced injury, and the protective effect of PCe is not mediated by interaction with adenosine triphosphate‑sensitive K+ channels. In the presence of PCe, production of ROS under chemical hypoxia was remarkably reduced which suggests that PCe might exert its effect as a ROS scavenger.Conclusion: The present study provides clear evidence for the beneficial effect of PCe on cardiomyocyte injury during hypoxia or reoxygenation following prolonged hypoxia.
Keywords: Cardiomyocyte; hypoxia; Pogostemon cablin
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