Quantitative analysis of the eight major compounds in the Samsoeum using a high‑performance liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometer

Abstract

Background: Samsoeum was traditionally used for treatment of a respiratory disease.Objective: The simultaneous determination of eight major compounds, ginsenoside Rg3, caffeic acid, puerarin, costunolide, hesperidin, naringin, glycyrrhizin, and 6‑gingerol in the Samsoeum using a high‑performance liquid chromatography (HPLC) coupled with diode array detection (DAD) and an electrospray ionization mass spectrometer was developed for an accurate and reliable quality assessment.Materials and Methods: Eight compounds were qualitative identified based on their mass spectra and by comparing with standard compounds and quantitative analyzed by HPLC‑DAD. Separation of eight compounds was carried out on a LUNA C18 column (S‑5 µm, 4.6 mm i.d. ×250 mm) with gradient elution composed of acetonitrile and 0.1% trifluoroacetic acid.Results: The data showed good linearity (R2 > 0.9996). The limits of detection and the limits of quantification were <0.53 μg and 1.62 μg, respectively. Inter‑ and Intra‑day precisions (expressed as relative standard deviation values) were within 1.94% and 1.91%, respectively. The recovery of the method was in the range of 94.24–107.90%.Conclusion: The established method is effective and could be applied to quality control of Samsoeum.

Keywords: High‑performance liquid chromatography‑diode array detection; high‑performance liquid chromatography‑mass spectrometer; marker constituents; samsoeum; simultaneous determination; validation