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PM · Lens · S29 Antimutagenic potential of harpagoside and Harpagophytum procumbens against 1‑nitropyrene

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Article · Volume 11, Issue 42S · pp. 29–36

S29 Antimutagenic potential of harpagoside and Harpagophytum procumbens against 1‑nitropyrene

Luigi Manon1, Baghdikian Béatrice2, Orsière Thierry1, Pompili Jocelyne1, Mabrouki Fathi2, Ollivier Evelyne1

  1. 1Botta Alain Department of Biogenotoxicology, Human Health and Environment, Mediterranean Institute of Biodiversity and Ecology, Faculty of Medicine, Aix‑Marseille University, 27 Bd Jean Moulin, France.
  2. 2Laboratory of Pharmacognosy and Ethnopharmacology, Faculty of Pharmacy, Aix‑Marseille University, 27 Bd Jean Moulin, Marseille Cedex 5, France.

Abstract

Background: 1‑nitropyrene (1‑NPy) is one of the most abundant nitro‑polycyclic aromatic hydrocarbons particularly in diesel exhausts. It is a mutagenic and carcinogenic pollutant very widespread in the environment. So the discovery of antimutagenic agents is essential. Harpagophytum procumbens (HP) is traditionally used as anti‑inflammatory and analgesic particularly against painful osteoarthritis. Harpagoside (HS), its major iridoid glycoside, is considered as the main active component.Objective: The aim of the present study was to evaluate the antimutagenic activity of HS and HP extracts against mutagenic activity of 1‑NPy.Materials and Methods: The antimutagenic activity was investigated using the in vitro cytokinesis‑block micronucleus assay in cultured human lymphocytes. Cells were exposed to HS or HP extracts before (pretreatment), during (co‑treatment), and after (posttreatment) treatment with 1‑NPy.Results: Results showed that HS significantly reduced the mutagenicity of 1‑NPy in pretreatment and particularly in co‑treatment, whereas all HP extracts significantly reduced the genotoxicity in the three protocols.Conclusion: These results suggested that HS was strongly involved in antimutagenic activity of HP extracts in co‑treatment, but other components in HP extracts participated in this activity in pre‑ and post‑treatment.

Keywords: 1‑nitropyrene; anti‑inflammatory; antimutagenic effect; cytokinesis‑block micronucleus assay; Harpagophytum procumbens; harpagoside

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