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PM · Lens · A direct protein kinase B‑targeted anti‑inflammatory activity of cordycepin from artificially cultured fruit body of Cordyceps militaris

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Article · Volume 11, Issue 43 · pp. 477–485

A direct protein kinase B‑targeted anti‑inflammatory activity of cordycepin from artificially cultured fruit body of Cordyceps militaris

Ju Young Yoon1, Ji Hye Kim1, Kwang‑Soo Baek1, Geum Soog Kim2, Seung Eun Lee2, Dae Young Lee2, Je Hun Choi2, Seung Yu Kim2, Hyun Bong Park3, Gi‑Ho Sung2, Kang Ro Lee3, Jae Youl Cho1, Hyung Jun Noh2

  1. 1Department of Genetic Engineering, Sungkyunkwan University, Suwon, Korea.
  2. 2Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseoung 369 873, Korea.
  3. 3Department of Pharmacy, School of Pharmacy, Sungkyunkwan University, Suwon 440 746.

Abstract

Background: Cordyceps militaris is one of well-known medicinal mushrooms with anti-inflammatory, anti-cancer, anti-diabetic, and anti-obesity activities.Objective: The objective of the following study is to isolate chemical components from the ethanol extract (Cm-EE) from Cordyceps militaris and to evaluate their anti-inflammatory activities.Materials and Methods: Column chromatographic separation was performed and anti-inflammatory roles of these compounds were also examined by using NO production and protein kinase B (AKT) activity assays.Results: From Cm-EE, 13 constituents, including trehalose (1), cordycepin (2), 6-hydroxyethyladenosine (3), nicotinic amide (4), butyric acid (5), β-dimorphecolic acid (6), α-dimorphecolic acid (7), palmitic acid (8), linoleic acid (9), cordycepeptide A (10), 4-(2-hydroxy-3-((9E,12E)-octadeca-9,12-dienoyloxy)propoxy)-2- (trimethylammonio)butanoate (11), 4-(2-hydroxy-3-(palmitoyloxy)propoxy)-2-(trimethylammonio) butanoate (12), and linoleic acid methyl ester (13) were isolated. Of these components, compound 2 displayed a significant inhibitory effect on NO production in lipopolysaccharide (LPS)-activated RAW264.7 cells. Furthermore, this compound strongly and directly suppressed the kinase activity of AKT, an essential signalling enzyme in LPS-induced NO production, by interacting with its ATP binding site.Conclusion: C. militaris could have anti-inflammatory activity mediated by cordycepin-induced suppression of AKT.

Keywords: Anti‑inflammatory activity; cordycepin; Cordyceps militaris (Clavicipitaceae); phytochemical study; protein kinase B A B S T R A C T

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