Article · Volume 12, Issue 45 · pp. 70–74
Optimization of Process Parameters and Kinetic Model of Enzymatic Extraction of Polyphenols from Lonicerae Flos
Fansheng Kong2,3,4, Shujuan Yu2,4, Yongguang Bi3, Xiaojun Huang3, Mengqian Huang3
- 1College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
- 2College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, China.
- 3Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, China.
Abstract
Objective: To optimize and verify the cellulase extraction of polyphenols from honeysuckle and provide a reference for enzymatic extracting polyphenols from honeysuckle.Materials and Methods: The uniform design was used According to Fick’s first law and kinetic model, fitting analysis of the dynamic process of enzymatic extracting polyphenols was conducted.Results: The optimum enzymatic extraction parameters for polyphenols from honeysuckle are found to be 80% (v/v) of alcohol, 35:1 (mL/g) of liquid-solid ratio, 80°C of extraction temperature, 8.5 of pH, 6.0 mg of enzyme levels, and 130 min of extraction time. Under the optimal conditions, the extraction rate of polyphenols was 3.03%. The kinetic experiments indicated kinetic equation ∞ ∞− 0 ln C C C had a good linear relationship with t even under the conditions of different levels of enzyme and temperature, which means fitting curve tallies well with the experimental values.Conclusion: The results of quantification showed that the results provide a reference for enzymatic extracting polyphenols from honeysuckle.
Keywords: Cellulase; kinetic model; Lonicerae Flos; polyphenols; uniform design
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