Article · Volume 12, Issue 45S · pp. S14–S20

Steroidal Saponin Diosgenin from Dioscorea bulbifera Protects Cardiac Cells from Hypoxia-reoxygenation Injury Through Modulation of Pro-survival and Pro-death Molecules

Karuppiah Shanmugasundarapandian Jayachandran¹, A. Hannah Rachel Vasanthi², Narasimman Gurusamy³

¹Department of Biotechnology, Centre for Advanced Research in Indian System of Medicine, SASTRA University, Thanjavur, Tamil Nadu, India.
²Department of Biotechnology, Pondicherry University, Puducherry, India.
³Department of Pharmacology, College of Pharmacy, King Khalid University, Abha, Kingdom of Saudi Arabia.

Abstract

Background: Diosgenin, a steroidal saponin from plants, exhibits many biological potentials. Herein, the cardioprotective role of diosgenin is studied.Materials and Methods: The effect of diosgenin, isolated from Dioscorea bulbifera, was studied on hypoxia-reoxygenation (HR) in H9c2 cardiomyoblast cells. The amount of diosgenin in the plant extract was analyzed by high-performance thin layer chromatography using a solvent system comprising of chloroform:methanol:acetic acid:formic acid (13:4.5:1.5:1). Cardioprotection was checked by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Further, the release of lactate dehydrogenase, an enzyme released during cell death was checked. The proteins responsible for cell death (Bax) and cell survival (Bcl-2, hemeoxygenase-1 and Akt) were analyzed using Western blot to check the cardioprotective role of diosgenin.Conclusion: Supplementation of diosgenin mitigates HR injury, thereby exhibiting cardioprotective potential.

Keywords: Cardioprotection; diosgenin; H9c2; hypoxia; reoxygenation

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