Article · Volume 12, Issue 46 · pp. 98–103
Quality Analysis of Chlorogenic Acid and Hyperoside in Crataegi fructus
Jin Bae Weon2, Youn Sik Jung2, Choong Je Ma2,3
- 1Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University.
- 2Department of Biomaterials Engineering, Kangwon National University, Chuncheon 200‑701, Korea.
Abstract
Background: Crataegi fructus is a herbal medicine for strong stomach, sterilization, and alcohol detoxification. Chlorogenic acid and hyperoside are the major compounds in Crataegi fructus.Objective: In this study, we established novel high‑performance liquid chromatography (HPLC)‑diode array detection analysis method of chlorogenic acid and hyperoside for quality control of Crataegi fructus.Materials and Methods: HPLC analysis was achieved on a reverse‑phase C18 column (5 μm, 4.6 mm × 250 mm) using water and acetonitrile as mobile phase with gradient system. The method was validated for linearity, precision, and accuracy. About 31 batches of Crataegi fructus samples collected from Korea and China were analyzed by using HPLC fingerprint of developed HPLC method. Then, the contents of chlorogenic acid and hyperoside were compared for quality evaluation of Crataegi fructus.Results: The results have shown that the average contents (w/w %) of chlorogenic acid and hyperoside in Crataegi fructus collected from Korea were 0.0438% and 0.0416%, respectively, and the average contents (w/w %) of 0.0399% and 0.0325%, respectively.Conclusion: In conclusion, established HPLC analysis method was stable and could provide efficient quality evaluation for monitoring of commercial Crataegi fructus.
Keywords: Crataegi fructus; chlorogenic acid; diode array detection; high performance liquid chromatography; hyperoside; quality evaluation
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