jrnls.org logo
Submit

BROWSE ALL JOURNALS

Type 2 letters to search all 19 journals

SEE ALL 19 JOURNALS
Pharmacognosy MagazineNOW IN
Article

Reversed Phase High‑Performance Liquid Chromatographic Ultra‑violet (Photo Diode Array) Quantification of Oleanolic Acid and its Isomer Ursolic Acid for Phytochemical Comparison and Pharmacological Evaluation of Four Leucas Species Used in Ayurveda

1, 1, 1★, 1★ Corresponding author

  1. 1Pharmacognosy and Ethnopharmacology Division, CSIR‑National Botanical Research Institute, Lucknow, Uttar Pradesh, India.

CORRESPONDENCE

Sharad Srivastava

Pharmacognosy and Ethnopharmacology Division, CSIR‑National Botanical Research Institute, Lucknow, Uttar Pradesh, India.

sharad_ks2003@yahoo.com

Received: 26-10-2015; Revised: 07-12-2015.

Volume 12, Issue 46S · pp. S159–S164 · 2016 · DOI: 10.4103/0973-1296.182173

View on PM original site ↗

ABSTRACT

Objective: Evaluation of four industrially important Leucas species for their in vitro antidiabetic potential and radical scavenging effect along with high‑performance liquid chromatographic quantification of the bioactive triterpenes.Materials and Methods: The quantification of triterpenes was carried out on C‑18 column with acetonitrile and water (90:10) as the solvent system at a detection wavelength of 210 nm. In vitro antidiabetic activity was evaluated by α‑amylase inhibition assay based on starch–iodine and 3,5 dinitrosalicylic acid (DNS) method. Antioxidant activity was calculated by five different models, namely total phenolic and total flavonoid content, free radical scavenging activity by 1‑1‑diphenyl‑2‑pic‑rylhydrazyl (DPPH), ferric‑reducing power assay, and the total antioxidant capacity.Results: Maximum concentration of oleanolic acid was found in Leucas cristata, followed by Leucas mollissima, Leucas Aspera, and Leucas biflora. Ursolic acid was highest in L. mollissima and then in L. biflora, L. cristata, and L. aspera, respectively. In in vitro antidiabetic activity, IC50 of L. aspera (1.56 ± 0.01 mg/ml) and L. mollissima (0.75 ± 0.005 mg/ml) were found to be highest in DNS and iodine starch assay. IC50 in DPPH assay ranges from 0.6 ± 0.011 to 1.68 ± 0.011 mg/ml. Antioxidant capacity follows the order; L. aspera > L. mollissima > L. biflora > L. cristata.Conclusion: Promising activities were observed in targeted species, thus L. mollissima, L. biflora, and L. cristata can be used alternatively as a substitute to L. aspera.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Shukla, P. K., Misra, A., Srivastava, S., & Rawat, A. K. S. (2016). Reversed Phase High‑Performance Liquid Chromatographic Ultra‑violet (Photo Diode Array) Quantification of Oleanolic Acid and its Isomer Ursolic Acid for Phytochemical Comparison and Pharmacological Evaluation of Four Leucas Species Used in Ayurveda. Pharmacognosy Magazine, 12(46S), S159–S164. https://doi.org/10.4103/0973-1296.182173