Article · Volume 12, Issue 46S · pp. S293–S296
A New Sucrase Enzyme Inhibitor from Azadirachta indica
Mohamed I. S. Abdelhady2,3, Usama Shaheen2,4, Ammar Bader2, Mahmoud A. Youns5,6
- 1Department of Pharmacognosy, Faculty of Pharmacy, Umm Al‑Qura University, Makkah 21955, Departments of, Saudi Arabia.
- 2Pharmacognosy and.
- 3Department of Pharmacognosy, Faculty of Pharmacy, Al‑Azhar University, Cairo, Egypt.
- 4Biochemistry, Faculty of Pharmacy, Helwan University, Cairo 11795, Egypt.
- 5Division of Functional Genome Analysis, German Cancer Research Center, Heidelberg, Germany.
Abstract
Background: Sucrase enzyme inhibitor considered as an oral anti-diabetic therapy that delays the absorption of eaten carbohydrates, reducing the postprandial glucose and insulin peaks to reach normoglycemia.Materials and Methods: Chromatographic fractionation of the hydroalcoholic extract of leaves of Azadirachta indica growing in KSA, followed by in-vitro assay of sucrase enzyme inhibition activity.Results: This investigation led to the isolation of a new remarkable sucrase enzyme inhibitor; 4`-methyl Quercetin-7-O-β -D-glucuronopyranoside (1) alongside with four known compounds; 2,3-hexahydroxydiphenoyl-(α/β)-D-4C1-glucopyranose (2), Avicularin (3), Castalagin (4) and Quercetin-3-O-glucoside (5). The structure of the new compound (1) was elucidated on the basis of its spectral data, including ESI-MS, UV, 1H NMR, 13C NMR, 1H-1H COSY, HSQC, NOESY and HMBC.Conclusion: Under the assay conditions, hydroalcoholic extract of A. indica and compounds 1-5 exhibited significant sucrase enzyme inhibitory activity.
Keywords: Azadirachta indica; polyphenols; sucrase inhibitor
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