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PM · Lens · Therapeutic Effect of Dendrobium candidum on Lupus Nephritis in Mice

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Article · Volume 13, Issue 49 · pp. 129–35

Therapeutic Effect of Dendrobium candidum on Lupus Nephritis in Mice

Qiang Wang1,2,3,4, Peng Sun1,2,3,4, Rui Wang1,2,3,4, Xin Zhao1,2,3,4

  1. 1Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing, P.R. China, China.
  2. 2Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing, P.R. China, China.
  3. 3Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing, P.R. China, China.
  4. 4Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing, P.R. China, China.

Abstract

Context: Dendrobium candidum (D.candimum) widely is a functional drug. The curative effect of D. candidum on lupus nephritis has been studied in vivo.Materials and Method: The DBA/2 and B6D2F1 mice were used for this in vivo experiment. The 50% effective dose (ED50) was used to check the effective concentration for this study. Then the SCr, BUN, TC, TG, IL-6, IL-12, TNF-α, and IFN-γ levels were determined by kits. The output of urine protein was determined by means of Coomassie Brilliant Blue, and the auto-antibody dsDNA was determined with titer plate technology and indirect immunofluorescence. The NF-κB, IκB-α, TGF‑β1, Fas, and FasL expressions were measured by RT-PCR and western blot assay. The component analysis of D. candidum was determined by nuclear magnetic resonance.Results: Based on the ED50 result at 329 mg/kg, 200 and 400 mg/kg doses were chosen for this study. SCr, BUN, TC and TG levels of 400 mg/kg D. candidum mice were lower than control mice, TP and ALB levels were higher than control mice. The control and 400 mg/kg treated mice tested positive for dsDNA at the end of sixth and tenth week after the experiment began. The glomerular number of 400 mg/kg treated mice was more than control group. Treatment with 400 mg/kg D. candidum reduced IL-6, IL-12, TNF-α and IFN-γcytokine levels as compared to control mice. D. candidum decreased NF-κb, TGF‑β1, Fas, FasL and increased IκB-α expressions in kidney tissue. There were 11 compounds in dry D. candidum, these compounds might make the curative effects of lupus nephritis.Conclusion: D. candidum showed a potential curative effect on lupus nephritis. It could be used as a health medicine on lupus nephritis.

Keywords: Cytokine; dendrobium candidum; inflammation; lupus nephritis; RT-PCR

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