Article · Volume 13, Issue 49 · pp. 175–179
Rapid Analysis of Components in Coptis chinensis Franch by Ultra-Performance Liquid Chromatography with Quadrupole Time-of-Flight Mass Spectrometry
Peng-peng Tian1,2, Xiao-xu Zhang1,2, Hong-ping Wang1, Pu-ling Li1,3, Yu-xin Liu2,4, Shao-jing Li1
- 1Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Dongzhimen, Beijing, China.
- 2Laboratory of Cell Pharmacology, College of Pharmaceutical Sciences, Hebei University, Baoding, China.
- 3Henan University of Traditional Chinese Medicine, Zhengzhou, China.
- 4Drug Quality Control Key Laboratory of Hebei Province, Hebei University, Baoding, China.
Abstract
Background: Coptis chinensis Franch is a traditional Chinese medical herb.Objective: In this article, ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used to rapidly, qualitatively, and comprehensively identify the components in Coptis chinensis Franch.Materials and Methods: Chromatographic separation was achieved on an Agilent Zorbax RRHD Eclipse Plus C18 column. The mobile phase consisted of 0.1% formic acid water (A) and 0.1% formic acid acetonitrile (B) with a gradient program. Qualitative analysis was performed on an Agilent 6540 quadrupole time-of-flight mass spectrometer, which was equipped with a Dual AJS ESI source operating in negative mode.Results: A total of 30 alkaloid and non-alkaloid components of Coptis chinensis Franch were identified in only 14 min.Conclusion: This study helped to provide a basis for the quality control of Coptis chinensis Franch.
Keywords: Alkaloids; Coptis chinensis Franch; non-alkaloids; Q-TOF; UPLC
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