Article · Volume 13, Issue 51S · pp. S481–S483
Chromosomal Fragmentation: A Possible Marker for the Selection of High Gymnemic Acid Yielding Accessions of Gymnema sylvestre R. Br
Ashutosh Kumar Verma1, Sunita Singh Dhawan2
- 1Central Botanical Laboratory, Botanical Survey of India, AJC Bose Indian Botanic Garden, Howrah, West Bengal, India.
- 2Plant Biotechnology Division, CSIR – Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh, India.
Abstract
Background: Gymnema sylvestre R. Br. a member of family Asclepiadaceae as mentioned in Indian Pharmacopoeia popular among the researchers because of stimulatory effect of its phytoconstituent on pancreatic cells and potential to treat Type I and II type of diabetes.Objectives: Development of cost‑effective marker system for the selection of high gymnemic acid yielding accessions of G. sylvestre.Materials and Methods: Presoaked seeds of Brassica campestris treated with different dilutions of gymnemagenin and 10% leaf extract of twenty different accessions of G. sylvestre. Root tips of germinated seeds were fixed, and chromosomal studies were made by root tip bioassay method.Results: Exposure of seeds to treatment solutions promotes various types of chromosomal anomalies in root meristem, and surprisingly, direct correlation between the percentage of chromosomal fragmentation and the percentage of gymnemic acid shared by treatment solution were observed.Conclusion: Later finding may be explored for the development of a novel methodology or marker system for the selection of high active principle yielding accessions of G. sylvestre.
Keywords: Accessions; chromosomal anomalies; Gymnema sylvestre; novel methodology
The full text of this article is available as a PDF. Open the PDF.