In vitro Cytotoxicity and Apoptotic Assay in HT‑29 Cell Line Using Ficus hispida Linn: Leaves Extract

Abstract

Background: Ficus hispida Linn. (Family Moraceae), well‑known beneficial medicinal shrub, has been traditionally used for the treatment of various diseases such as leukoderma.Objective: The aim of the present study is to investigate the efficacy of F. hispida ethanolic leaves extract for antiproliferative, apoptotic, cell cycle blockade, and wound healing.Materials and Methods: F. hispida leaves extract was treated with colorectal adenocarcinoma cancer cell line HT29 for 24 h with control. The cells were treated at varying concentration ranges of 15, 31, 62, 125, and 250 µg/ml each The cytotoxicity effect of leaves extract was studied by 3‑[4, 5‑dimethylthiazol‑2‑yl]‑2, 5 diphenyltetrazolium bromide assay and their anticancer activity was further evaluated using cell cycle analysis and wound scratch assay.Results: The end antiproliferative result showed that HT‑29 cell viability decreases in a concentration‑dependent manner and the growth inhibitory effect (IC50) values are obtained at a concentration of 125 μg. The increase in number of apoptotic cell was observed after treating HT‑29 cells with the sample in double‑staining methods. G0/G1 phase of the cell cycle was significantly blocked by the test sample followed by the G2/M phase in a negligible manner. In vitro cell wound closure or contracture was not significant when compared the sample against control group.Conclusion: F. hispida Linn. ethanolic leaves extract had shown to possess excellent cytotoxic effect through inducing apoptosis, especially causing cell cycle arrest at the G0/G1 phase.

Keywords: Apoptosis; cancer; cytotoxicity; Ficus hispida; phytochemicals; plant extract