Article · Volume 13, Issue 52S · pp. S822–S827
Expression of Hepatic Cytochrome P450s in Rats Administered with Guibi-tang, a Traditional Herbal Formula
Seong Eun Jin2, Hyekyung Ha2, Chang‑Seob Seo2, Hyeun‑Kyoo Shin2, Soo‑Jin Jeong3,4
- 1K‑herb Research Center, Korea Institute of Oriental Medicine.
- 2KM Convergence Research Division, Korea Institute of Oriental Medicine.
- 3Korean Medicine Life Science, University of Science and Technology, Daejeon, Republic of Korea.
Abstract
Objective: The aim of this study was to investigate the possible herb-drug interactions between the traditional herbal formula Guibi‑tang (GBT; Guipi‑tang, Kihi‑to) and conventional drugs.Materials and Methods: GBT was orally administered to either male or female Sprague Dawley (SD) rats once daily at doses of 1000, 2000, or 5000 mg/kg/day for 13 weeks. The messenger ribonucleic acid (mRNA) expression of drug‑metabolizing enzyme cytochrome P450 isozymes (cytochrome P450s; CYP1A1, 1A2, 2B1/2, 2C11, 2E1, 3A1, 3A2, and 4A1) was analyzed in hepatic tissues by reverse transcription‑polymerase chain reaction.Results: Repeated oral administration of GBT did not significantly influence the mRNA expression of hepatic CYP1A1, 1A2, 2B1/2, 2C11, 2E1, 3A1, 3A2, and 4A1 in male rats. By contrast, in female rats, the mRNA expression of hepatic CYP1A2 and 2B1/2 was significantly increased by repeated GBT treatment.Conclusion: Our findings indicate that caution is required in females when GBT is taken concomitantly with conventional drugs metabolized by CYP1A2 or 2B1/2. Our results provide information regarding the safety and effectiveness of GBT for clinical use.
Keywords: Cytochrome P450; Guibi‑tang; herb–drug interactions; rat liver
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