Article · Volume 15, Issue 60 · pp. 156–163

Optimization of Ultrasonic‑Assisted Extraction of Bioactive Compounds from Sargassum henslowianum using Response Surface Methodology

Yongguang Bi^2,3, Yanshan Lu², Hong Yu², Lili Luo²

¹Department of Pharmaceutical Engineering, College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, China.
²State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Hainan, Haikou, China.

Abstract

Objective: This study was to optimize the ultrasound‑assisted extraction of polysaccharides and polyphenols from S. henslowianum and test the biological activity of the extract.Materials and Methods: Response surface methodology was used to optimize extraction conditions, and the antioxidant activity of the extracts was evaluated by radical scavenging assay, α‑Glucosidase inhibition, and cytotoxicity on MCF‑7.Results: The optimal conditions of extracting polysaccharides were shown as following: ultrasonic time for 40 min, ultrasonic power for 330 W, solid‑to‑liquid ratio for 1:36 g/mL, the extraction yield reached 12.63% under above parameters. The optimum conditions of ultrasonic‑assisted extraction of total polyphenols were as following: ultrasonic time for 102 min, ultrasonic power for 377 W, alcohol concentration for 62%, under these conditions, the extraction yield reached 11.45%. Besides, the extracts of polyphenols possessed stronger activity.Conclusion: This study provides the scientific guidance for further exploitation and utilization of S. henslowianum.

Keywords: Bioactivity; response surface methodology; Sargassum henslowianum; ultrasonic extraction

The full text of this article is available as a PDF. Open the PDF.