Anti‑Inflammatory Activity of Corylopsis coreana Uyeki Extracts and Isolated Compounds by Inhibiting Nitric Oxide and Cytokine Production

Abstract

Aims: To study the anti‑inflammatory effects of C. coreana Uyeki leaf and bark extracts as well as their major active compounds, we evaluated in vitro levels of tumor necrosis factor‑α (TNF‑α), interleukin‑6 (IL‑6), and nitric oxide (NO) using RAW 264.7 macrophages.Materials and Methods: Production of TNF‑α and IL‑6 cytokines was measured by ELISA assay, and NO inhibitory activity was tested by Griess assay.Results: Extracts of leaves and barks, as well as isolated compounds from these extracts, showed remarkable anti‑inflammatory activities by inhibiting NO, TNF‑α, and IL‑6 cytokine production. In particular, the leaf extract showed more potent inhibitory activities than that of barks.Conclusions: Ellagitannins and flavonoids were probably related to the anti‑inflammatory activity of CL (leaves) extract, providing evidence of the anti‑inflammatory property of CL.

Keywords: Anti‑inflammatory ability; Corylopsis coreana uyeki; cytokine production inhibition; interleukin‑6; nitric oxide production inhibition; tumor necrosis factor‑α