Article · Volume 15, Issue 62S · pp. S30–S33

In vitro Evaluation of the Effects of Lycopene on Caspase System and Oxidative DNA Damage in High‑Glucose Condition

Bunyamin Bazyel¹, Semiha Dede², Sedat Cetin², Veysel Yuksek³, Mehmet Taşpinar⁴

¹Health Science High School, Muş Alparslan University, Mus, Turkey.
²Department of Biochemistry, Veterinary Medical Faculty, Van Yuzuncu Yil University, Turkey.
³Ozalp Vocational High School, Van Yuzuncu Yil University, Turkey.
⁴Department of Medical Biology, Faculty of Medicine, Van Yuzuncu Yil University, Van, Turkey.

Abstract

Background: The present study was planned to investigate the effects of lycopene, on the caspase‑dependent apoptosis in high‑dose glucose (HG)‑treated PC12 cell line. PC12 cells were cultured in vitro.Materials and Methods: HG was prepared as G (250 mM), and lycopene was prepared as L1 (10 μM), L2 (20 μM), and L3 (40 μM). After 6 h of incubation, the cells were exposed to trypsin, and the samples were obtained with freeze/ thaw method. Caspase 3, 8, 9; 8‑hydroxy‑2‑deoxyguanosine (8‑OHdG); and M30 were determined (enzyme‑linked immunosorbent assay).Results: 8‑OHdG increased in L3 (P ≤ 0.001), whereas L1 caused a decrease in HG group (P ≤ 0.001). Caspase‑3 decreased significantly in L1, L2, and L3G compared to control (P ≤ 0.001) group. Caspase‑8 increased significantly in L1, L1G, L2G, and all L3 glucose groups (P ≤ 0.001). There was no difference for Caspase‑9. M30 was not affected by L and HG, which decreased significantly (P ≤ 0.001).Conclusion: As a result, it was determined that, when PC12 cell line was treated with HG, lycopene application had effects on caspase enzymes and DNA damage.

Keywords: Caspases; high glucose; in vitro; lycopene; oxidative DNA damage

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