Anti‑Eczematic and Molecular Modeling of Anthraquinones Isolated from the Seeds of Asphodelus microcarpus Salzm. Viv. Growing in Egypt

Abstract

Objective: The objective of the study is to investigate the anti‑eczematic activity, acute toxicity, and molecular modeling of A. microcarpus seeds.Materials and Methods: Nuclear magnetic resonance, ultraviolet, and mass spectroscopy were applied for characterization of isolated metabolites; induction of eczema was conducted by 2% and 0.2% w/v dinitrochlorobenzene in acetone; eczema was treated with topical application of the different seed extracts in the form of ointments (1% w/w); Swiss albino mice (25–30 g) were used for the determination of LD50 and anti‑eczematic effect. Docking studies were performed by Molecular Operating Environment software.Results: A. microcarpus seed extract exhibited promising ant‑eczematic activity, six anthraquinones were isolated from chloroform portion and characterized as 10,7`‑bichrysophanol (1), asphodelin (2), chrysophanol‑8‑O‑methyl ether (3), chrysophanol (4), physcion (5), and emodin (6). Compounds 1, 3, and 5 exerted significant anti‑eczematic effect.Conclusion: Six known anthraquinone derivatives were isolated and characterized for the first time from the seeds of A. microcarpus. Chloroform fraction (1% w/w) showed significant anti‑eczematic effect compared to standard mometasone furoate (0.1 w/w). The docking study proved the anti‑eczematic activity of anthraquinone content by their affinity to the target human histamine H1 receptor.

Keywords: Anthraquinones; Asphodelus microcarpus; docking; eczema; Xanthorrhoeaceae