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PM · Lens · Cellular Protection Induced by Genistein in Mouse and its Antioxidant Capacity

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Article · Volume 15, Issue 66S · pp. S520–S526

Cellular Protection Induced by Genistein in Mouse and its Antioxidant Capacity

Rogelio Paniagua-Pérez1, Susana Reyes-Cadena1, Carlos Martínez-Canseco1, Celia Reyes-Legorreta1, Jesús Martínez-Castro2, Eduardo O. Madrigal-Santillán3, José A. Morales-González3, José M. Cristóbal-Luna4, Isela Álvarez-González4, Eduardo Madrigal-Bujaidar4

  1. 1Department of Biochemistry, National Institute of Rehabilitation.
  2. 2Department of Computer, Computer Research Center, National Polytechnic Institute.
  3. 3Department of Graduate, Conservation Medicine Laboratory, National Polytechnic Institute.
  4. 4Department of Morphology, Genetics Laboratory, National Polytechnic Institute, Mexico City, México.

Abstract

Objective: The main objective of the study is to determine the capacity of GT to inhibit the genotoxic and cytotoxic damage induced by CP in mouse, as well as its immunostimulant ability and its capacity to scavenge free radicals.Materials and Methods: We determined the effect of six doses of GT on the rate of sister chromatid exchanges (SCEs) and of micronuclei (MN) in mice administered with 5 mg/kg of CP. Besides, we determined its capacity to increase the amount of lymphocytes in mouse and to reduce oxidation with the 2,2‑diphenyl‑1‑picrylhydrazyl assay.Results: Our results showed that GT (10–60 mg/kg) significantly decreased the frequency of SCE and of MN in mice. Furthermore, we also observed a moderate bone marrow cytotoxic correction of the damage induced by CP, as shown by an improvement in the rate of polychromatic erythrocytes. In addition, with 60 mg/kg, GT increased 69.6% the production of mouse lymphocytes over the control value throughout a 72‑h trial. Moreover, the compound also showed a high capacity to trap free radicals (95.25%, with 250 µg/ml).Conclusion: Our results, therefore, established that GT is an effective cellular protective agent against the action of CP.

Keywords: 2; 2‑diphenyl‑1‑picrylhydrazyl; cisplatin; genistein; lymphocytes; micronuclei; sister chromatid exchange

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