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PM · Lens · Chromatographic Investigation of Phytoconstituents in Milk Yam (Ipomoea digitata L.) Tubers

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Article · Volume 16, Issue 5S · pp. S462–S466

Chromatographic Investigation of Phytoconstituents in Milk Yam (Ipomoea digitata L.) Tubers

N. S. Sonia1, Thomas George2

  1. 1Department of Plantation Crops and Spices, College of Agriculture, India.
  2. 2Pesticide Residue Research and Analytical Laboratory, College of Agriculture, Thiruvananthapuram, Kerala, India.

Abstract

Objective: The present study is done with the aim of identifying the maturity stage of milk yam tuber containing maximum concentration of umbelliferone – a coumarin present in the tubers.Materials and Methods: Umbelliferone present in immature tubers (6 months after planting), overmature tubers (36 months after planting), and tubers at optimum maturity (21 months after planting) was assessed using high‑performance liquid chromatography (HPLC) and high‑performance thin‑layer chromatography (HPTLC) analysis.Results: HPLC and HPTLC techniques revealed the presence of umbelliferone and it was quantified to be higher in over mature tubers (0.44 and 0.42 µg/g, respectively), followed by optimally mature (0.31 and 0.22 µg/g, respectively) and immature tubers (0.20 and <0.11 µg/g, respectively).Conclusion: The study reveales that developmental stages affect umbelliferone concentration in milk yam tubers and it increased as the plant ages.

Keywords: Development stages; high‑performance liquid chromatography; high‑performance thin‑layer chromatography; phytochemical; umbelliferone

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