Effect of Vitex pinnata L. Leaf Extract on Estrogenic Activity and Lipid Profile in Ovariectomized Rats

Abstract

Objectives: To compare the estrogenic activity and lipid profile of ethanol extracts of leaves of Vitex pinnata L. with 17 β‑estradiol in bilaterally ovariectomized (OVX) rats.Methods: Ethanol extracts were analyzed by gas chromatography‑mass spectrometry (GC‑MS). Bilaterally OVX rats were divided into five groups,(n = 6) receiving different treatments, consisting of a vehicle (1% Tween), ethanol extract of V. pinnata at three different doses (100, 500, 1000 mg/kg) and standard drug, 17 β‑estradiol at a dose of 1 mg/kg. All groups were administered orally, daily for 14 days.Results: GC‑MS data revealed that the major chemical constituents of the extract were 3, 7, 11, 15‑Tetramethylhexadecen‑2‑en‑1‑ol, Gamma‑Stigmasterol, 9,12,15‑octadecatrienoic acid and n‑hexadecanoic acid. V. pinnata extracts at 1000 mg/kg slightly increased uterine and vaginal weight and endometrial thickness. Doses of extract at 500 and 1000 mg/kg induced a significant (P < 0.05) decrease of triglycerides and total cholesterol in serum of OVX rats.Conclusion: V. pinnata leaf extract exhibits estrogenic activity and reduces levels of serum triglycerides and cholesterol. The understanding of such activity of V. pinnata leaf extract has benefits for postmenopausal women.

Keywords: 17 β‑estradiol; estrogenic activity; lipid profile; ovariectomized rats; Vitex pinnata