Article · Volume 16, Issue 67 · pp. 148–155
A Rapid and Efficient Approach Based on Ultra‑High Liquid Chromatography Coupled with Mass Spectrometry for Identification in vitro and in vivo Constituents from Shizao Decoction
Yu-Mei Wang1, Qi Liu1, Wen-Hao Fu1, Ai-Hua Zhang2
- 1Qiqihar Academy of Medical Sciences, The Research Institute of Medicine and Pharmacy, Qiqihar Medical University, Qiqihar, Heilongjiang, China.
- 2National Chinmedomics Research Center, Sino‑America Chinmedomics Technology Collaboration Center, National Traditional Chinese Medicine Key Laboratory of Serum Pharmacochemistry, Metabolomics Laboratory, Heilongjiang University of Chinese Medicine, Harbin, China.
Abstract
Background: The genus <em>Cissus</em> (Vitaceae) is known for its medicinal properties. <em>Cissus quadrangularis</em> is a well-known medicinal plant used in traditional medicine for bone healing.Objective: The present study was aimed to evaluate the anti-inflammatory and analgesic activities of the methanolic extract of <em>Cissus quadrangularis</em> (MECQ) in experimental animal models.Materials and Methods: The anti-inflammatory activity was evaluated using carrageenan-induced rat paw edema model, and analgesic activity was evaluated using acetic acid-induced writhing and hot plate methods in mice.Results: MECQ at doses of 200 and 400 mg/kg showed significant (P < 0.01) anti-inflammatory activity in carrageenan-induced rat paw edema. In analgesic models, MECQ exhibited significant (P < 0.01) reduction in the number of writhes in acetic acid-induced writhing test and increased the reaction time in hot plate method.Conclusion: The results suggest that MECQ possesses significant anti-inflammatory and analgesic activities, which supports its traditional use.
Keywords: Constituents; decoction; mass spectrometry; traditional Chinese medicine; ultra‑high liquid chromatography
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