Extraction, Isolation and Identification of Kaempferol 3,7 – Diglucoside in the Leaf Extracts of Evolvulus alsinoides (Linn.) and its Inhibition Potency against α‑Amylase, α‑Glucosidase, Acetylcholinesterase and Amyloid Aggregation

Abstract

Objectives: To report the enzyme inhibitory potency of the plant extract and to isolate the compound of significant impact.Materials and Methods: The plant extracts were obtained by serial exhaustive extraction and successive chromatographic separation. Antioxidant and enzyme inhibition assays were carried out to extract the required isolate.Results: The current research identifies for the first time, the presence of Kaempferol 3,7 diglycoside in the leaf extracts by Spectrophotometry, Chromatography, 1H and 13C Nuclear Magnetic Resonance, carboxylic and hydroxylic hydrolysis. The isolated and characterized compound possesses enzyme inhibition property towards α‑amylase, α‑glucosidase and Acetylcholinesterase.Conclusion: The compound protects differentiating neuronal cells, SH‑SY5Y from Amyloid β Peptide‑induced Injury. Thus, the newly identified compound could serve as a plant‑based bioactive in the management of Alzheimer’s.

Keywords: Evolvulus alsinoides (Linn.); kaempferol 3; 7-diglucoside; enzyme inhibition; neuroblastoma cell lines; spectral analysis