jrnls.org logo
Submit

BROWSE ALL JOURNALS

Type 2 letters to search all 19 journals

SEE ALL 19 JOURNALS
Pharmacognosy MagazineNOW IN
Back

PM · Lens · Protein Expression Patterns in HEK‑Blue™ ‑ Cells Treated with Clinacanthus nutans Extracts

PDF

Article · Volume 16, Issue 70S · pp. S431–S434

Protein Expression Patterns in HEK‑Blue™ ‑ Cells Treated with Clinacanthus nutans Extracts

Shun‑Kai Yang2, Teik‑Kee Leo3, Chun‑Wai Mai4,5, Swee‑Yee Chin4, Dhilia Udie Lamasudin2, Swee‑Hua‑Erin Lim6, Ngai‑Paing Tan7, Kok‑Song Lai6

  1. 1Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia.
  2. 2Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, Selangor, School of Pharmacy, International Medical University.
  3. 3Department of Pharmaceutical Chemistry, School of Pharmacy, International Medical University.
  4. 4Centre for Cancer and Stem Cells Research, Institute for Research, Development and Innovation, International Medical University, Kuala Lumpur.
  5. 5Health Sciences Division, Abu Dhabi Women’s College, Higher Colleges of Technology, Abu Dhabi, United Arab Emirates.
  6. 6Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, Selangor, School of Pharmacy, International Medical University, Malaysia.

Abstract

Objectives: This study aimed to investigate the effect of CN extract on human embryonic kidney cell line (HEK‑Blue™‑4) in a proteomic perspective.Materials and Methods: Comparative proteomic profiling through two‑dimensional sodium dodecyl sulfate gel electrophoresis was performed on HEK‑Blue™‑4 treated with CN leaf polar extract.Results: We successfully identified seven upregulated proteins, of which five promoted the growth of the HEK‑Blue™‑4 cells. Interestingly, a potent antioxidant enzyme which neutralizes reactive oxygen or nitrogen species, peroxiredoxin‑1 was also upregulated in HEK‑Blue™‑4 cell lines after treatment with CN leaf polar extract.Conclusion: CN leaf polar extract promotes the growth of HEK‑Blue™‑4 cells and induced the expression of peroxiredoxin‑1, which protects the cells from reactive oxygen species during the inflammation process.

Keywords: Anti‑inflammatory; Clinacanthus nutans; comparative proteomic; HEK‑Blue™‑4 cell line; two‑dimensional sodium dodecyl sulfate gel electrophoresis

The full text of this article is available as a PDF. Open the PDF.