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PM · Lens · Honey as a Solvent for the Green Extraction, Analysis, and Bioconversion of Daidzin from Pueraria candollei var. mirifica Root

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Article · Volume 16, Issue 71 · pp. 524–530

Honey as a Solvent for the Green Extraction, Analysis, and Bioconversion of Daidzin from Pueraria candollei var. mirifica Root

Suppalak Phaisan2, Gorawit Yusakul2, Poomraphie Nuntawong3, Seiichi Sakamoto3, Waraporn Putalun4,5, Satoshi Morimoto3, Hiroyuki Tanaka6

  1. 1Department of Industrial Pharmacy, School of Pharmacy, Walailak University.
  2. 2Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812‑8582, Japan.
  3. 3Department of Pharmacognosy and Toxicology, Faculty of Pharmaceutical Sciences, Khon Kaen University.
  4. 4Research Group for Pharmaceutical Activities of Natural Products Using Pharmaceutical Biotechnology, National Research University‑Khon Kaen University, Khon Kaen 40002, Thailand.
  5. 5Department of Pharmacognosy and Kampo, Faculty of Pharmaceutical Sciences, Sanyo‑Onoda City University, Yamaguchi, Japan.

Abstract

Objectives: This study aims to apply honey (H‑NADES) and a sugar‑based NADES (S‑NADES) for the extraction, analysis, and bioconversion of daidzin from Pueraria candollei var. mirifica (PM) root.Materials and Methods: Various concentrations of H‑NADES and S‑NADES (water:sucrose:glucose: fructose, 18:3:18:22 by weight) were used as solvents for extraction. Indirect competitive enzyme‑linked immunosorbent assay (icELISA) was developed and validated for monitoring the extraction efficacy. The catalytic reactivity against daidzin of β‑glucosidase purified from honey was investigated.Results: Using NADESs as solvents, icELISA was suitable for the reliable determination of daidzin with high sensitivity (1.95–125 ng/mL), specificity (% cross‑reactivity ≤ 2.60), and accuracy (98.3‑106% daidzin recovery). Daidzin at a concentration of 75.8 ± 3.67 µg/mL was extracted using 50% (v/v) S‑NADES, which was the most effective for the extraction compared to H‑NADES, water and ethanol. In addition, daidzin was converted to daidzein by honey β‑glucosidase.Conclusion: Both S‑NADES and H‑NADES were useful for the extraction, analysis, and bioconversion of daidzin, and β‑glucosidase from honey might enhance the oestrogenic activity and bioavailability of PM phytochemicals.

Keywords: Enzyme‑linked immunosorbent assay; daidzein; daidzin; honey; Pueraria mirifica

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