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PM · Lens · Chlorogenic Acid in Viscum album Callus is a Potential Anticancer Agent against C6 Glioma Cells

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Article · Volume 16, Issue 71 · pp. 531–537

Chlorogenic Acid in Viscum album Callus is a Potential Anticancer Agent against C6 Glioma Cells

Jinwoo Kim1, Suji Baek1, Kang Pa Lee1, Byung Seok Moon4, Hyun-Soo Kim5, Seung-Hae Kwon6, Dae won Lee7, Jisu Kim8,9

  1. 1Department of Gyeongsangbuk-do Arboretum, Sumogwon-ro 647, Pohang, Gyeongbuk (37502).
  2. 2Research and Development Center, UMUST R&D Corporation, Neungdong-ro, Gwangjin-gu, Seoul (05029).
  3. 3Department of Nuclear Medicine, Ewha Womans University Seoul Hospital, Ewha Womans University College of Medicine, Seoul (07804).
  4. 4National Marine Biodiversity Institute of Korea, Jangsan-ro, Seocheon, Chungcheongnam-do (33662), Republic of Korea.
  5. 5Korea Basic Science Institute, Seoul, (02841).
  6. 6Department of Bio-Science, College of Natural Science, Dongguk University, Dongdae-ro, Gyeongju, Gyeongbuk (38066).
  7. 7Physical Activity & Performance Institute, Konkuk University, Neungdong-ro, Gwangjin-gu, Seoul (05029).
  8. 8Department of Sports Medicine and Science in Graduated School, Konkuk University, Neungdong-ro, Gwangjin-gu, Seoul (05029), Republic of Korea.

Abstract

Objective: Our study aimed to explore the anticancer effects of CA obtained from Viscum album callus on C6 glioma cell migration and proliferation.Materials and Methods: Anticancer potency was analyzed by the 2,3‑bis‑(2‑methoxy‑4‑nitro‑5‑sulfophenyl)‑2H‑tetrazolium‑5‑carboxanilide salt assay to assess the ability to inhibit cell growth and proliferation. Cell mobility was investigated based on the Boyden chamber and the scratch wound healing assay. Factors involved in cell cycle progression were evaluated by mRNA and protein expression. Cell death was determined by staining with specific dyes and fluorescence microscopy.Results: CA significantly reduced C6 glioma cell proliferation and migration. Furthermore, it induced reactive oxygen species generation and apoptotic cell death. Treatment with CA also suppressed extracellular signal‑regulated kinase ½ (ERK½) phosphorylation and the gene expression of cyclins E and A.Conclusion: Our results show that CA may regulate glioma cell migration and proliferation via modulation of ERK½ phosphorylation and cell cycle regulation. Thus, it might be a potent anticancer agent in preventing progression of glioma.

Keywords: Apoptosis; chlorogenic acid; extracellular signal‑regulated kinase ½; glioma; proliferation

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