Article · Volume 17, Issue 74 · pp. 231–235
Costunolide Attenuates Oxygen‑glucose Deprivation/ Reoxygenation‑Induced Apoptosis in Mouse Brain Slice through Inhibiting Caspase Expression
Huixia Ma2, Yafei Zhu2,4, Zhengjun Zhang5, Xinhui Zhang2,3, Qipeng Zhao2,3
- 1Department of Pharmacology, School of Pharmacy, Ningxia Medical University, China.
- 2Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education, Ningxia Medical University, China.
- 3College of Basic Medicine, Ningxia Medical University, China.
- 4Department of Cardiology, General Hospital of Ningxia Medical University, Yinchuan, China.
Abstract
Background: Costunolide (Co) has anti‑tumor, anti‑inflammation, and anti‑ulcer effects, it has the budding effect of anti‑apoptosis. This study was intended to explicate its inhibitory effect on caspase in a mice brain slices injury model.Materials and Methods: The maestro 11.1 software was employed to envisage the binding sites of Co with Caspase-3, Caspase‑9, and Caspase‑7. Oxygen‑glucose deprivation/reoxygenation (OGD/R) method was employed to persuade mouse brain slice injury in vitro. Purpose of lactate dehydrogenase (LDH) in culture medium and 2,3,5‑triphenyl‑tetrazolium chloride (TTC) staining of brain slices for the assessment of injury degree. The expression of Cytochrome c, Caspase‑9, Caspase‑7, Caspase‑3, Bcl‑2, and Bax was studied by Western blot method.Results: The results of docking displayed that Co had binding sites withe Caspase‑9, Caspase‑7, and Caspase‑3. Compared with OGD/R, Co could diminution the LDH levels, upsurge the TTC staining intensity, augment Bcl‑2 expression level and inhibit Caspase‑3, Caspase‑9, Caspase‑7, Bax, and Cytochrome c expression levels.Conclusion: These results recommended that Co has latent neuroprotective activities by inhibiting caspase expression.
Keywords: Apoptosis; brain slice; Caspase-3; costunolide; ischemic stroke
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