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PM · Lens · In vitro Genotoxicity and in vivo Single‑Dose Oral Toxicity of Polysaccharide Fraction from the Leaves of Diospyros kaki Thunb.

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Article · Volume 17, Issue 75 · pp. 406–412

In vitro Genotoxicity and in vivo Single‑Dose Oral Toxicity of Polysaccharide Fraction from the Leaves of Diospyros kaki Thunb.

Chang-Won Cho2, Young-Ran Song2,3, Youn-Hwan Hwang4, Young Kyoung Rhee2, Won-Chul Lim2, Jae Woong Choi2, Kyung-Tae Lee5, Hee-Do Hong2

  1. 1Research Group of Traditional Food, Korea Food Research Institute, Wanju, Jeonbuk 55365.
  2. 2Foundation of Agricultural Technology Commercialization and Transfer, Iksan 54667.
  3. 3Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 34504.
  4. 4Department of Pharmaceutical Biochemistry, Kyung Hee University, Seoul 02447, Republic of Korea.

Abstract

Background: Polysaccharides isolated from medicinal herbs are regarded as important bioactive materials due to their various physiological activities. In our previous study, the polysaccharide fraction (PLE0) was separated from pectinase-digested persimmon (Diospyros kaki Thunb.) leaves and showed immunostimulatory and anti‑osteoporotic effects. However, there is currently no information regarding the toxicity of PLE0.Materials and Methods: As a toxicological evaluation, acute oral toxicity and in vitro genotoxicity assays (chromosomal aberration and bacterial reverse mutation tests) were performed.Results: In the single‑dose acute oral toxicity test in female and male Sprague‑Dawley (SD) rats, the median lethal dose of PLE0 was higher than 5000 mg/kg and adverse effects were not observed in terms of mortality and abnormal changes in clinical signs. Furthermore, chromosomal abnormality and bacterial reverse mutation tests showed that PLE0 displays no mutagenicity or clastogenicity.Conclusion: Based on these results, PLE0 was not mutagenic and did not induce chromosomal aberration in vitro under our experimental conditions and exhibited no acute oral toxicity at up to 5000 mg/kg in SD rats.

Keywords: Acute oral toxicity; bacterial reverse mutation; chromosomal aberration; Diospyros kaki Thunb. leaves; polysaccharide

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