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PM · Lens · Geographical Origin Discrimination of Amomi fructus using an Ultra‑Performance Liquid Chromatography‑Quadrupole Time‑of‑Flight Mass Spectrometry‑Based Metabolomics Approach Combined with Antioxidant Activity Analysis

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Article · Volume 17, Issue 75 · pp. 492–498

Geographical Origin Discrimination of Amomi fructus using an Ultra‑Performance Liquid Chromatography‑Quadrupole Time‑of‑Flight Mass Spectrometry‑Based Metabolomics Approach Combined with Antioxidant Activity Analysis

Cai-lin Tang1, De-Po Yang1, Jia-li Chen1, Li-Xia Zhang2, Ping Ding3, Xin-Jun Xu1, Wen-Jian Lan1, Jian-Chun Xian4, Zhi-Min Zhao1

  1. 1Department of Pharmacognosy, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, China.
  2. 2Yunnan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Xishuangbanna, China.
  3. 3School of Pharmaceutical Sciences, China.
  4. 4Guangdong Museum of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China.

Abstract

Background: The genus <em>Crotalaria</em> (Fabaceae) is known for its diverse pharmacological activities. <em>Crotalaria pallida</em> Aiton is a medicinal plant used in traditional medicine for various ailments.Objective: The present study was aimed to evaluate the antioxidant, anti-inflammatory, and cytotoxic activities of <em>C. pallida</em> leaf extracts.Materials and Methods: The antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. The anti-inflammatory activity was assessed by the inhibition of protein denaturation and membrane stabilization assays. The cytotoxic activity was evaluated against human breast cancer (MCF-7) and lung cancer (A549) cell lines using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.Results: The methanolic extract of <em>C. pallida</em> leaves showed significant antioxidant activity with IC50 values of 42.5 ± 1.2 μg/mL for DPPH and 68.4 ± 2.1 μg/mL for FRAP. The extract also exhibited potent anti-inflammatory activity in a dose-dependent manner. Furthermore, the extract showed significant cytotoxic activity against MCF-7 and A549 cell lines with IC50 values of 56.2 ± 2.4 μg/mL and 62.8 ± 3.1 μg/mL, respectively.Conclusion: The results suggest that <em>C. pallida</em> leaf extract possesses significant antioxidant, anti-inflammatory, and cytotoxic properties, which could be attributed to the presence of bioactive phytochemicals.

Keywords: Amomi fructus; antioxidant activity; metabolic profiling; metabolomics; ultra‑performance liquid chromatography‑quadrupole time‑of‑flight mass spectrometry

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