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PM · Lens · Anti‑Inflammatory Effects of Malus toringoides Extract in Lipopolysaccharide‑Induced Human Umbilical Vein Endothelial Cells

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Article · Volume 17, Issue 75 · pp. 518–524

Anti‑Inflammatory Effects of Malus toringoides Extract in Lipopolysaccharide‑Induced Human Umbilical Vein Endothelial Cells

Chengde Fan2, Zhuoma Dongzhi3,4, Linsha Dong2, Ruiying Yuan3,5, Jule Wang3, Bin Li2, Shan Huang2

  1. 1Department of Pharmacy, Key Laboratory of Pharmaceutical Research for Metabolic Diseases, Qingdao University of Science and Technology, Qingdao.
  2. 2Department of Medicament, College of Medicine, Tibet University, Lhasa, China.
  3. 3Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla, Thailand.
  4. 4Center of Tibetan Studies (Everest Research Institute), Tibet University, Lhasa, China.

Abstract

Objectives: The aim of the study was to explore the anti‑inflammatory effects and cellular mechanisms of extracts of M. toringoides (CBTM‑E375) in lipopolysaccharide (LPS)‑induced human umbilical vein endothelial cells (HUVECs).Materials and Methods: HUVECs were exposed to LPS, and the level of proinflammatory mediators was measured by enzyme‑linked immunosorbent assay. Furthermore, the activation of heme oxygenase‑1 (HO‑1), nuclear factor erythroid 2‑related factor 2 (Nrf2), and mitogen‑activated protein kinase (MAPK) was examined by Western blot and immunofluorescence analysis.Results: CBTM‑E375 significantly downregulated the levels of inflammatory mediators and upregulated the expression of HO‑1 by modulating Nrf2 translocation in HUVECs. The transfection of HO‑1 small interfering RNA into HUVECs actively reversed the effects of CBTM‑E375 in suppressing the expression of proinflammatory cytokines. Furthermore, MAPK activation in response to LPS was also blocked by CBTM‑E375.Conclusion: CBTM‑E375 exerts anti‑inflammatory effects, possibly by modulating the translocation of Nrf2 and expression of HO‑1, and inhibiting the phosphorylation of MAPK signaling pathway.

Keywords: Human umbilical vein endothelial cells; Malus toringoides (Rehd.) Hughes; mitogen‑activated protein kinases; nuclear factor erythroid 2‑related factor 2/heme oxygenase‑1

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