Article · Volume 17, Issue 76 · pp. 666–672
Phytochemical Investigation of Bauhinia winitii Based on Alpha‑Glucosidase Inhibitory Effect and Molecular Docking Affirmation
Sukanya Dej‑adisai2, Ichwan Ridwan Rais2,3, Chatchai Wattanapiromsakul2, Thanet Pitakbut4
- 1Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Songkhla, Thailand.
- 2Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Ahmad Dahlan, Yogyakarta, Indonesia.
- 3Department of Biochemical and Chemical Engineering, Technical University of Dortmund, Dortmund, Germany.
Abstract
Objectives: The aim of this study was to investigate the phytochemical constituents and anti‑alpha glucosidase activity of BW.Materials and Methods: Phytochemical study was based on the alpha‑glucosidase inhibitory effect. Isolated compounds were determined by chromatographic and spectroscopic techniques. The alpha‑glucosidase enzymatic inhibitory test was then connected with molecular docking affirmation.Results: The ethyl acetate and ethanol extracts from leaves and woods exhibited the strongest activity with above 60% inhibition. Six compounds were isolated from these extracts, naringenin (1), luteolin (2), isoquercitrin (3), griffonilide (4), lithospermoside (5), and epi‑β amyrin (6). The alpha‑glucosidase inhibitory test showed that flavonoids performed potential results with naringenin as the highest IC50 on 0.41 mM. The docking ensured the flavonoids activated at the same binding of alpha‑glucosidase enzyme active site.Conclusion: This study would be the first report of chemical constituents from BW with its isolated compounds was presented anti‑diabetic activity as alpha‑glucosidase inhibitors.
Keywords: Alpha‑glucosidase inhibitor; anti‑diabetes; Bauhinia winitii; molecular docking; phytochemical investigation
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